Dataset Information

A foldamer-dendrimer conjugate neutralizes synaptotoxic ?-amyloid oligomers.

ABSTRACT:

Background and aims

Unnatural self-organizing biomimetic polymers (foldamers) emerged as promising materials for biomolecule recognition and inhibition. Our goal was to construct multivalent foldamer-dendrimer conjugates which wrap the synaptotoxic ?-amyloid (A?) oligomers with high affinity through their helical foldamer tentacles. Oligomeric A? species play pivotal role in Alzheimer's disease, therefore recognition and direct inhibition of this undruggable target is a great current challenge.

Methods and results

Short helical ?-peptide foldamers with designed secondary structures and side chain chemistry patterns were applied as potential recognition segments and their binding to the target was tested with NMR methods (saturation transfer difference and transferred-nuclear Overhauser effect). Helices exhibiting binding in the µM region were coupled to a tetravalent G0-PAMAM dendrimer. In vitro biophysical (isothermal titration calorimetry, dynamic light scattering, transmission electron microscopy and size-exclusion chromatography) and biochemical tests (ELISA and dot blot) indicated the tight binding between the foldamer conjugates and the A? oligomers. Moreover, a selective low nM interaction with the low molecular weight fraction of the A? oligomers was found. Ex vivo electrophysiological experiments revealed that the new material rescues the long-term potentiation from the toxic A? oligomers in mouse hippocampal slices at submicromolar concentration.

Conclusions

The combination of the foldamer methodology, the fragment-based approach and the multivalent design offers a pathway to unnatural protein mimetics that are capable of specific molecular recognition, and has already resulted in an inhibitor for an extremely difficult target.

SUBMITTER: Fulop L

PROVIDER: S-EPMC3408453 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

A foldamer-dendrimer conjugate neutralizes synaptotoxic β-amyloid oligomers.

Fülöp Lívia L Mándity István M IM Juhász Gábor G Szegedi Viktor V Hetényi Anasztázia A Wéber Edit E Bozsó Zsolt Z Simon Dóra D Benkő Mária M Király Zoltán Z Martinek Tamás A TA

PloS one 20120730 7

<h4>Background and aims</h4>Unnatural self-organizing biomimetic polymers (foldamers) emerged as promising materials for biomolecule recognition and inhibition. Our goal was to construct multivalent foldamer-dendrimer conjugates which wrap the synaptotoxic β-amyloid (Aβ) oligomers with high affinity through their helical foldamer tentacles. Oligomeric Aβ species play pivotal role in Alzheimer's disease, therefore recognition and direct inhibition of this undruggable target is a great current cha ...[more]

PMID: 22859942

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