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Separation of variant methylated histone tails by differential ion mobility.

ABSTRACT: Differential ion mobility spectrometry (field asymmetric waveform ion mobility spectrometry (FAIMS)) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here, we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in the middle-down range.

SUBMITTER: Shvartsburg AA

PROVIDER: S-EPMC3418378 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Separation of variant methylated histone tails by differential ion mobility.

Shvartsburg Alexandre A AA Zheng Yupeng Y Smith Richard D RD Kelleher Neil L NL

Analytical chemistry 20120718 15

Differential ion mobility spectrometry (field asymmetric waveform ion mobility spectrometry (FAIMS)) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here, we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in t ...[more]

PMID: 22812477

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