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Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies.

ABSTRACT: Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8(+) conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry.

SUBMITTER: Giam M

PROVIDER: S-EPMC3434660 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies.

Giam M M Mintern J D JD Rautureau G J P GJ Hinds M G MG Strasser A A Bouillet P P

Cell death & disease 20120823

Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the ...[more]

PMID: 22914326

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