Ontology highlight
ABSTRACT:
SUBMITTER: Shioi G
PROVIDER: S-EPMC3466090 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Shioi Go G Kiyonari Hiroshi H Abe Takaya T Nakao Kazuki K Fujimori Toshihiko T Jang Chuan-Wei CW Huang Cheng-Chiu CC Akiyama Haruhiko H Behringer Richard R RR Aizawa Shinichi S
Genesis (New York, N.Y. : 2000) 20110617 7
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demons ...[more]