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Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain.

ABSTRACT: The ability to image neurons anywhere in the mammalian brain is a major goal of optical microscopy. Here we describe a minimally invasive microendoscopy system for studying the morphology and function of neurons at depth. Utilizing a guide cannula with an ultrathin wall, we demonstrated in vivo two-photon fluorescence imaging of deeply buried nuclei such as the striatum (2.5 mm depth), substantia nigra (4.4 mm depth) and lateral hypothalamus (5.0 mm depth) in mouse brain. We reported, for the first time, the observation of neuronal activity with subcellular resolution in the lateral hypothalamus and substantia nigra of head-fixed awake mice.

SUBMITTER: Bocarsly ME

PROVIDER: S-EPMC4646561 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain.

Bocarsly Miriam E ME Jiang Wan-Chen WC Wang Chen C Dudman Joshua T JT Ji Na N Aponte Yeka Y

Biomedical optics express 20151023 11

The ability to image neurons anywhere in the mammalian brain is a major goal of optical microscopy. Here we describe a minimally invasive microendoscopy system for studying the morphology and function of neurons at depth. Utilizing a guide cannula with an ultrathin wall, we demonstrated in vivo two-photon fluorescence imaging of deeply buried nuclei such as the striatum (2.5 mm depth), substantia nigra (4.4 mm depth) and lateral hypothalamus (5.0 mm depth) in mouse brain. We reported, for the fi ...[more]

PMID: 26601017

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