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1?,25-dihydroxyvitamin D3 promotes CD200 expression by human peripheral and airway-resident T cells.

ABSTRACT: BACKGROUND:CD200, a cell-surface immunoglobulin-like molecule expressed by immune and stromal cells, dampens the pro-inflammatory activity of tissue-resident innate cells via its receptor, CD200R. This interaction appears critical for peripheral immune tolerance, particularly in the airways where excessive inflammation is undesirable. Vitamin D contributes to pulmonary health and promotes regulatory immune pathways, therefore its influence on CD200 and CD200R was investigated. METHODS:CD200 and CD200R expression were assessed by qPCR and immunoreactivity of human lymphoid, myeloid and epithelial cells following 1?,25-dihydroxyvitamin D3 (1?,25VitD3) exposure in vitro and in peripheral T cells following 1?,25VitD3 oral ingestion in vivo. The effect of 1?25VitD3 was also assessed in human airway-resident cells. RESULTS:1?25VitD3 potently upregulated CD200 on peripheral human CD4+ T cells in vitro, and in vivo there was a trend towards upregulation in healthy, but not asthmatic individuals. CD200R expression was not modulated in any cells studied. CD200 induction was observed to a lesser extent in CD8+ T cells and not in B cells or airway epithelium. T cells isolated from the human airway also responded strongly to 1?25VitD3 to upregulate CD200. CONCLUSIONS:The capacity of 1?,25-dihydroxyvitamin D3 to induce CD200 expression by peripheral and respiratory tract T cells identifies an additional pathway via which vitamin D can restrain inflammation in the airways to maintain respiratory health.

SUBMITTER: Dimeloe S

PROVIDER: S-EPMC3471129 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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1α,25-dihydroxyvitamin D3 promotes CD200 expression by human peripheral and airway-resident T cells.

Dimeloe Sarah S Richards David F DF Urry Zoe L ZL Gupta Atul A Stratigou Victoria V Farooque Sophie S Saglani Sejal S Bush Andrew A Hawrylowicz Catherine M CM

Thorax 20120214 7

<h4>Background</h4>CD200, a cell-surface immunoglobulin-like molecule expressed by immune and stromal cells, dampens the pro-inflammatory activity of tissue-resident innate cells via its receptor, CD200R. This interaction appears critical for peripheral immune tolerance, particularly in the airways where excessive inflammation is undesirable. Vitamin D contributes to pulmonary health and promotes regulatory immune pathways, therefore its influence on CD200 and CD200R was investigated.<h4>Methods ...[more]

PMID: 22334534

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Dataset Information

1?,25-dihydroxyvitamin D3 promotes CD200 expression by human peripheral and airway-resident T cells.

Publications

1α,25-dihydroxyvitamin D3 promotes CD200 expression by human peripheral and airway-resident T cells.

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