Ontology highlight
ABSTRACT:
SUBMITTER: Chng SS
PROVIDER: S-EPMC3489181 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Chng Shu-Sin SS Xue Mingyu M Garner Ronald A RA Kadokura Hiroshi H Boyd Dana D Beckwith Jonathan J Kahne Daniel D
Science (New York, N.Y.) 20120830 6102
The presence of lipopolysaccharide (LPS) on the cell surface of Gram-negative bacteria is critical for viability. A conserved β-barrel membrane protein LptD (lipopolysaccharide transport protein D) translocates LPS from the periplasm across the outer membrane (OM). In Escherichia coli, this protein contains two disulfide bonds and forms the OM LPS translocon with the lipoprotein LptE. Here, we identified seven in vivo states on the oxidative-folding pathway of LptD. Proper assembly involved a no ...[more]