Ontology highlight
ABSTRACT:
SUBMITTER: Richler E
PROVIDER: S-EPMC3523716 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Nature methods 20071216 1
We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The method has several advantages over previous attempts to image P2X channel activation by fluorescence resonance energy transfer (FRET): notably, it reports channel opening rather than ...[more]