Unknown

Dataset Information

0

Generation and characterization of a lysosomally targeted, genetically encoded Ca(2+)-sensor.


ABSTRACT: Distinct spatiotemporal Ca2+ signalling events regulate fundamental aspects of eukaryotic cell physiology. Complex Ca2+ signals can be driven by release of Ca2+ from intracellular organelles that sequester Ca2+ such as the ER (endoplasmic reticulum) or through the opening of Ca2+-permeable channels in the plasma membrane and influx of extracellular Ca2+. Late endocytic pathway compartments including late-endosomes and lysosomes have recently been observed to sequester Ca2+ to levels comparable with those found within the ER lumen. These organelles harbour ligand-gated Ca2+-release channels and evidence indicates that they can operate as Ca2+-signalling platforms. Lysosomes sequester Ca2+ to a greater extent than any other endocytic compartment, and signalling from this organelle has been postulated to provide 'trigger' release events that can subsequently elicit more extensive Ca2+ signals from stores including the ER. In order to investigate lysosomal-specific Ca2+ signalling a simple method for measuring lysosomal Ca2+ release is essential. In the present study we describe the generation and characterization of a genetically encoded, lysosomally targeted, cameleon sensor which is capable of registering specific Ca2+ release in response to extracellular agonists and intracellular second messengers. This probe represents a novel tool that will permit detailed investigations examining the impact of lysosomal Ca2+ handling on cellular physiology.

SUBMITTER: McCue HV 

PROVIDER: S-EPMC3526116 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Generation and characterization of a lysosomally targeted, genetically encoded Ca(2+)-sensor.

McCue Hannah V HV   Wardyn Joanna D JD   Burgoyne Robert D RD   Haynes Lee P LP  

The Biochemical journal 20130101 2


Distinct spatiotemporal Ca2+ signalling events regulate fundamental aspects of eukaryotic cell physiology. Complex Ca2+ signals can be driven by release of Ca2+ from intracellular organelles that sequester Ca2+ such as the ER (endoplasmic reticulum) or through the opening of Ca2+-permeable channels in the plasma membrane and influx of extracellular Ca2+. Late endocytic pathway compartments including late-endosomes and lysosomes have recently been observed to sequester Ca2+ to levels comparable w  ...[more]

Similar Datasets

| S-EPMC3615648 | biostudies-literature
| S-EPMC4557345 | biostudies-literature
| S-EPMC5700382 | biostudies-literature
| S-EPMC3560286 | biostudies-literature
| S-EPMC7404130 | biostudies-literature
| S-EPMC2614750 | biostudies-literature
| S-EPMC3846734 | biostudies-literature
| S-EPMC8544647 | biostudies-literature
| S-EPMC7659708 | biostudies-literature
| S-EPMC3593221 | biostudies-literature