Ontology highlight
ABSTRACT:
SUBMITTER: Hadavi R
PROVIDER: S-EPMC3558152 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Avicenna journal of medical biotechnology 20100401 2
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as E ...[more]