Unknown

Dataset Information

0

E2A transcription factors limit expression of Gata3 to facilitate T lymphocyte lineage commitment.


ABSTRACT: The E2A transcription factors promote the development of thymus-seeding cells, but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. Here, we showed that E2A proteins were required to promote T-lymphocyte commitment from DN2 thymocytes and to extinguish their potential for alternative fates. E2A proteins functioned in DN2 cells to limit expression of Gata3, which encodes an essential T-lymphocyte transcription factor whose ectopic expression can arrest T-cell differentiation. Genetic, or small interfering RNA-mediated, reduction of Gata3 rescued T-cell differentiation in the absence of E2A and restricted the development of alternative lineages by limiting the expanded self-renewal potential in E2A?/? DN2 cells. Our data support a novel paradigm in lymphocyte lineage commitment in which the E2A proteins are necessary to limit the expression of an essential lineage specification and commitment factor to restrain self-renewal and to prevent an arrest in differentiation.

SUBMITTER: Xu W 

PROVIDER: S-EPMC3587319 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

E2A transcription factors limit expression of Gata3 to facilitate T lymphocyte lineage commitment.

Xu Wei W   Carr Tiffany T   Ramirez Kevin K   McGregor Stephanie S   Sigvardsson Mikael M   Kee Barbara L BL  

Blood 20130107 9


The E2A transcription factors promote the development of thymus-seeding cells, but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. Here, we showed that E2A proteins were required to promote T-lymphocyte commitment from DN2 thymocytes and to extinguish their potential for alternative fates. E2A proteins functioned in DN2 cells to limit expression of Gata3, which encodes an essential T-lymphocyte transcription factor whose ectopic expressi  ...[more]

Similar Datasets

| S-EPMC4767349 | biostudies-literature
| S-EPMC11226602 | biostudies-literature
| S-EPMC2663971 | biostudies-literature
| S-EPMC3925327 | biostudies-literature
| S-EPMC6962539 | biostudies-literature
| S-EPMC2190753 | biostudies-literature
| S-EPMC3311756 | biostudies-literature
2016-04-06 | E-GEOD-76348 | biostudies-arrayexpress
| S-EPMC4500571 | biostudies-literature
| S-EPMC2196077 | biostudies-literature