Ontology highlight
ABSTRACT:
SUBMITTER: Huang L
PROVIDER: S-EPMC3650468 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Huang Lan L Marvin Julie M JM Tatsis Nia N Eisenlohr Laurence C LC
Journal of immunology (Baltimore, Md. : 1950) 20110114 4
The importance of ubiquitination in MHC class I-restricted Ag processing remains unclear. To address this issue, we overexpressed wild-type and dominant-negative lysineless forms of ubiquitin (Ub) in mammalian cells using an inducible vaccinia virus system. Overexpression of the lysineless Ub nearly abrogated polyubiquitination and potently inhibited epitope presentation from a cytosolic N-end rule substrate as well as endoplasmic reticulum (ER)-targeted model Ags. In contrast, there was little ...[more]