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A cost effective real-time PCR for the detection of adenovirus from viral swabs.


ABSTRACT: Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.

SUBMITTER: Al-Siyabi T 

PROVIDER: S-EPMC3679997 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A cost effective real-time PCR for the detection of adenovirus from viral swabs.

Al-Siyabi Turkiya T   Binkhamis Khalifa K   Wilcox Melanie M   Wong Sallene S   Pabbaraju Kanti K   Tellier Raymond R   Hatchette Todd F TF   LeBlanc Jason J JJ  

Virology journal 20130607


Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method show  ...[more]

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