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A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR.

ABSTRACT: Real-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effective approach for preparing and assembling cDNA synthesis and real-time PCR mastermixes with similar efficiencies as commercially available kits. Our results thus demonstrate an alternative to commercially available kits.

SUBMITTER: Mote RD

PROVIDER: S-EPMC8626763 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR.

Mote Ridim D RD Laxmikant V Shinde VS Singh Surya Bansi SB Tiwari Mahak M Singh Hemant H Srivastava Juhi J Tripathi Vidisha V Seshadri Vasudevan V Majumdar Amitabha A Subramanyam Deepa D

Journal of biosciences 20210101

Real-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effe ...[more]

PMID: 34845993

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