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High-resolution analysis of cis-acting regulatory networks at the ?-globin locus.

ABSTRACT: We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human ?-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the ?-globin cluster.

SUBMITTER: Hughes JR

PROVIDER: S-EPMC3682726 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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High-resolution analysis of cis-acting regulatory networks at the α-globin locus.

Hughes Jim R JR Lower Karen M KM Dunham Ian I Taylor Stephen S De Gobbi Marco M Sloane-Stanley Jacqueline A JA McGowan Simon S Ragoussis Jiannis J Vernimmen Douglas D Gibbons Richard J RJ Higgs Douglas R DR

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20130506 1620

We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a ...[more]

PMID: 23650635

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Comprehensive, high resolution analysis of cis-acting regulatory networks at the alpha globin locus

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