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A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions.

ABSTRACT: Studies of protein-protein interactions deep in organs and in whole mammals have been hindered by a lack of genetically encoded fluorescent probes in near-infrared region for which mammalian tissues are the most transparent. We have used a near-infrared fluorescent protein iRFP engineered from a bacterial phytochrome as the template to develop an in vivo split fluorescence complementation probe. The domain architecture-based rational design resulted in an iSplit reporter with the spectra optimal for whole-body imaging, high photostability, and high complementation contrast, which compares favorably to that of other available split fluorescent protein-based probes. Successful visualization of interaction of two known protein partners in a living mouse model suggests iSplit as the probe of choice for noninvasive detection of protein-protein interactions in vivo, whereas its fast intracellular degradation enables time-resolved monitoring of repetitive binding events.

SUBMITTER: Filonov GS

PROVIDER: S-EPMC3757571 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions.

Filonov Grigory S GS Verkhusha Vladislav V VV

Chemistry & biology 20130725 8

Studies of protein-protein interactions deep in organs and in whole mammals have been hindered by a lack of genetically encoded fluorescent probes in near-infrared region for which mammalian tissues are the most transparent. We have used a near-infrared fluorescent protein iRFP engineered from a bacterial phytochrome as the template to develop an in vivo split fluorescence complementation probe. The domain architecture-based rational design resulted in an iSplit reporter with the spectra optimal ...[more]

PMID: 23891149

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