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A nanopore machine promotes the vectorial transport of DNA across membranes.

ABSTRACT: The transport of nucleic acids through membrane pores is a fundamental biological process that occurs in all living organisms. It occurs, for example, during the import of viral DNA into the host cell or during the nuclear pore complex-mediated transport of mRNA in and out the cell nucleus and has implications in nucleic acid drug delivery and gene therapy. Here we describe an engineered DNA transporter that is able to recognize and chaperone a specific DNA molecule across a biological membrane under a fixed transmembrane potential. The transported DNA strand is then released by a simple mechanism based on DNA strand displacement. This nanopore machine might be used to separate or concentrate nucleic acids or to transport genetic information across biological membranes.

SUBMITTER: Franceschini L

PROVIDER: S-EPMC3778508 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A nanopore machine promotes the vectorial transport of DNA across membranes.

Franceschini Lorenzo L Soskine Misha M Biesemans Annemie A Maglia Giovanni G

Nature communications 20130101

The transport of nucleic acids through membrane pores is a fundamental biological process that occurs in all living organisms. It occurs, for example, during the import of viral DNA into the host cell or during the nuclear pore complex-mediated transport of mRNA in and out the cell nucleus and has implications in nucleic acid drug delivery and gene therapy. Here we describe an engineered DNA transporter that is able to recognize and chaperone a specific DNA molecule across a biological membrane ...[more]

PMID: 24026014

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