Ontology highlight
ABSTRACT:
SUBMITTER: Kupi T
PROVIDER: S-EPMC3824300 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
European biophysics journal : EBJ 20130815 10
Formins are highly conserved proteins that are essential in the formation and regulation of the actin cytoskeleton. The formin homology 2 (FH2) domain is responsible for actin binding and acts as an important nucleating factor in eukaryotic cells. In this work EPR and DSC were used to investigate the properties of the mDia1-FH2 formin fragment and its interaction with actin. MDia1-FH2 was labeled with a maleimide spin probe (MSL). EPR results suggested that the MSL was attached to a single SH gr ...[more]