Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC3880544 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Wang Jiadong J Chen Junjie J Gong Zihua Z
Molecular cell 20131114 5
Human TopBP1 is a key mediator protein involved in DNA replication checkpoint control. In this study, we report a specific interaction between TopBP1 and Bloom syndrome helicase (BLM) that is phosphorylation and cell-cycle dependent. Interestingly, TopBP1 depletion led to decreased BLM protein level and increased sister chromatid exchange (SCE). Moreover, our data indicated that BLM was ubiquitinated by E3 ligase MIB1 and degraded in G1 cells but was stabilized by TopBP1 in S phase cells. Deplet ...[more]