Ontology highlight
ABSTRACT:
SUBMITTER: Choi Y
PROVIDER: S-EPMC3914775 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20120101 6066
Tethering a single lysozyme molecule to a carbon nanotube field-effect transistor produced a stable, high-bandwidth transducer for protein motion. Electronic monitoring during 10-minute periods extended well beyond the limitations of fluorescence techniques to uncover dynamic disorder within a single molecule and establish lysozyme as a processive enzyme. On average, 100 chemical bonds are processively hydrolyzed, at 15-hertz rates, before lysozyme returns to its nonproductive, 330-hertz hinge m ...[more]