Ontology highlight
ABSTRACT:
SUBMITTER: Choi Y
PROVIDER: S-EPMC3750062 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Physical chemistry chemical physics : PCCP 20130901 36
Single molecule bioelectronic circuits provide an opportunity to study chemical kinetics and kinetic variability with bond-by-bond resolution. To demonstrate this approach, we examined the catalytic activity of T4 lysozyme processing peptidoglycan substrates. Monitoring a single lysozyme molecule through changes in a circuit's conductance helped elucidate unexplored and previously invisible aspects of lysozyme's catalytic mechanism and demonstrated lysozyme to be a processive enzyme governed by ...[more]