Ontology highlight
ABSTRACT:
SUBMITTER: Braberg H
PROVIDER: S-EPMC3932829 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Braberg Hannes H Jin Huiyan H Moehle Erica A EA Chan Yujia A YA Wang Shuyi S Shales Michael M Benschop Joris J JJ Morris John H JH Qiu Chenxi C Hu Fuqu F Tang Leung K LK Fraser James S JS Holstege Frank C P FC Hieter Philip P Guthrie Christine C Kaplan Craig D CD Krogan Nevan J NJ
Cell 20130808 4
RNA polymerase II (RNAPII) lies at the core of dynamic control of gene expression. Using 53 RNAPII point mutants, we generated a point mutant epistatic miniarray profile (pE-MAP) comprising ∼60,000 quantitative genetic interactions in Saccharomyces cerevisiae. This analysis enabled functional assignment of RNAPII subdomains and uncovered connections between individual regions and other protein complexes. Using splicing microarrays and mutants that alter elongation rates in vitro, we found an inv ...[more]