Ontology highlight
ABSTRACT:
SUBMITTER: Abdur R
PROVIDER: S-EPMC3940196 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Acta crystallographica. Section D, Biological crystallography 20140129 Pt 2
The crystal structures of protein-nucleic acid complexes are commonly determined using selenium-derivatized proteins via MAD or SAD phasing. Here, the first protein-nucleic acid complex structure determined using selenium-derivatized nucleic acids is reported. The RNase H-RNA/DNA complex is used as an example to demonstrate the proof of principle. The high-resolution crystal structure indicates that this selenium replacement results in a local subtle unwinding of the RNA/DNA substrate duplex, th ...[more]