Dataset Information

Association of G894T polymorphism in endothelial nitric oxide synthase gene with the risk of ischemic stroke: A meta-analysis.

ABSTRACT: Findings of a previous meta-analysis demonstrated no association between G894T polymorphism in endothelial nitric oxide synthase (eNOS) gene and ischemic stroke. Results of other studies have also been inconsistent. Updated meta-analysis was performed to assess the association between the eNOS gene G894T (rs1799983) polymorphism and the risk of ischemic stroke. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) from fixed and random effects models were calculated. Heterogeneity in the studies was evaluated using the I2. Meta-regression and the 'leave-one-out' sensitive analysis were used to examine the potential sources of between-study heterogeneity. Publication bias was estimated using the Egger's test. Data were available for 6,607 cases and 6,947 controls from 22 studies. Studies deviating from the Hardy-Weinberg equilibrium (HWE) in the controls and the key contributors to between-study heterogeneity were excluded. Significant associations between eNOS gene G894T polymorphism and the risk of ischemic stroke were observed in the dominant (OR 1.249; 95% CI, 1.145-1.361), the recessive (OR 1.255; 95% CI, 1.082-1.456) and the codominant models (OR 1.195; 95% CI, 1.115-1.281). Moreover, in the subgroup analysis based on the region (Asia and Europe), significant associations were observed between the dominant and codominant genetic models but not in the recessive model. The results of our meta-analysis suggested that eNOS gene G894T polymorphism was associated with the increased risk of ischemic stroke, and that the T allele may be an important risk factor for ischemic stroke. However, further studies are required to confirm this result.

SUBMITTER: Wang M

PROVIDER: S-EPMC3956621 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Association of G894T polymorphism in endothelial nitric oxide synthase gene with the risk of ischemic stroke: A meta-analysis.

Wang Meiyun M Jiang Xiubo X Wu Wenlong W Zhang Dongfeng D

Biomedical reports 20121016 1

Findings of a previous meta-analysis demonstrated no association between G894T polymorphism in endothelial nitric oxide synthase (eNOS) gene and ischemic stroke. Results of other studies have also been inconsistent. Updated meta-analysis was performed to assess the association between the eNOS gene G894T (rs1799983) polymorphism and the risk of ischemic stroke. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) from fixed and random effects models were calculated. Heterogeneity in the ...[more]

PMID: 24648911

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Project description:Psoriasis is a common autoimmune-mediated chronic, inflammatory skin disease. Although, the molecular mechanism is not completely understood, psoriasis is caused by genetic and non-genetic parameters. The current study aimed (1) to define genotype and allele frequency of endothelial nitric oxide synthase (eNOS Glu298Asp) gene polymorphism in hypertensive and/or non-hypertensive psoriatic patients (2) to investigate the possible relationship between the eNOS Glu298Asp polymorphism and the risk of hypertension among psoriatic patients in the Turkish population.This cross-sectional, case-control study was performed between March 2010 and November 2012 at the University hospital in Çanakkale, Turkey Patients and Methods: Gene profiles of 75 psoriatic patients (21 hypertensive and 54 normotensive pa.tients) and 55 healthy (normotensive and non-psoriatic) volunteers were compared. Peripheral blood-EDTA samples were used for total genomic DNA isolation and genotyping. Target eNOS gene was genotyped for patients and control groups by real-time PCR melting-curve analysis system (LightCycler 2.0,Roche, Germany, and results were compared statistically.An increased T allele frequency in eNOS Glu298Asp single-nucleotide polymorphism (SNP) was determined in psoriatic patients when compared with normotensive non-psoriatic healthy volunteers (OR 2.3, CI 1.14-3.99), (P=.017). The T allele frequency was also found to be increased in hypertensive psoriatic patients when compared with healthy volunteers (4.83-fold increased, 95% CI 1.62-14.43 ([P=.003]) and normotensive psoriatic patients (3.03-fold increased, 95% CI 1.03-8.94 [P=.041]), respectively.The current preliminary results suggested that there was a correlation between eNOS Glu298Asp polymorphism and hypertension among psoriatic patients in the Turkish population. The T allele frequency of eNOS Glu298Asp SNP was different in hypertensive psoriatic patients, and the difference was statistically significant when compared with the normotensive psoriatic patients and healthy controls. These results need to be confirmed by large-scale studies.

Project description:AIM:To investigate the role of endothelial nitric oxide synthase -786T > C promoter polymorphism in the etiology of gastric cancer (GC). METHODS:A total of 150 GC patients and 150 control subjects were included in the study. The information on demographic features was elicited with an informed consent from all the patients and control subjects using a structured questionnaire. Helicobacter pylori (H. pylori) infectivity status was tested in antral biopsies from all the subjects by rapid urease test following the method of Vaira et al. Genomic DNA was isolated from whole blood samples following the salting out method of Lahiri et al. Genotype analysis of the rs2070744 polymorphism was carried out by allele-specific polymerase chain reaction method. The genotypes were determined based on the appearance of bands on an agarose gel stained with ethidium bromide under ultraviolet gel documentation with the help of 100 bp ladder. Odds ratios and corresponding 95%CIs were determined using java stat online software. RESULTS:There was a significant difference in the distribution of C allele (C vs T; P = 0.000, OR = 5.038) in patient group compared to the control subjects exhibiting a fivefold increased risk for GC. When the T/T and C/C genotypes were compared, there was an enhanced GC risk for individuals with C/C genotype (T/T vs C/C; P = 0.000). Among the demographic factors, smoking and alcoholism were the common risk factors in patients compared to the control subjects (P < 0.05). Patients with smoking and alcoholism developed cancer even in heterozygous T/C condition (smoking: P = 0.020 and alcoholism: P = 0.005). Individuals with H. pylori infection showed seven fold increased risk for cancer. All the patients with C/C genotype revealed a significant association between H. pylori infection and GC. Among the patients 2.4% of them revealed familial incidence of GC. No significant difference was noticed between cases and controls with regard to consanguinity (P = 0.473). CONCLUSION:The Present data suggest that eNOS-786 C/C genotype and C allele may be considered as potential risk factors in patients with GC.

Dataset Information

Association of G894T polymorphism in endothelial nitric oxide synthase gene with the risk of ischemic stroke: A meta-analysis.

Publications

Association of G894T polymorphism in endothelial nitric oxide synthase gene with the risk of ischemic stroke: A meta-analysis.

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