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A luminescent oxygen channeling biosensor that measures small GTPase activation.

ABSTRACT: We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.

SUBMITTER: Niebel B

PROVIDER: S-EPMC3961041 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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A luminescent oxygen channeling biosensor that measures small GTPase activation.

Niebel Björn B Weiche Benjamin B Mueller Alan L AL Li Dean Y DY Karnowski Nora N Famulok Michael M

Chemical communications (Cambridge, England) 20110531 26

We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts. ...[more]

PMID: 21625685

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