In vitro high throughput screening, what next? Lessons from the screening for aurora kinase inhibitors.
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ABSTRACT:
SUBMITTER: Hoang TM
PROVIDER: S-EPMC4009756 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
In vitro high throughput screening, what next? Lessons from the screening for aurora kinase inhibitors.
Hoang Thi-My-Nhung TM Vu Hong-Lien HL Le Ly-Thuy-Tram LT Nguyen Chi-Hung CH Molla Annie A
Biology 20140227 1
Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS. ...[more]
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