Ontology highlight
ABSTRACT:
SUBMITTER: Tenedini E
PROVIDER: S-EPMC4017260 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Tenedini E E Bernardis I I Artusi V V Artuso L L Roncaglia E E Guglielmelli P P Pieri L L Bogani C C Biamonte F F Rotunno G G Mannarelli C C Bianchi E E Pancrazzi A A Fanelli T T Malagoli Tagliazucchi G G Ferrari S S Manfredini R R Vannucchi A M AM Tagliafico E E
Leukemia 20131022 5
With the intent of dissecting the molecular complexity of Philadelphia-negative myeloproliferative neoplasms (MPN), we designed a target enrichment panel to explore, using next-generation sequencing (NGS), the mutational status of an extensive list of 2000 cancer-associated genes and microRNAs. The genomic DNA of granulocytes and in vitro-expanded CD3+T-lymphocytes, as a germline control, was target-enriched and sequenced in a learning cohort of 20 MPN patients using Roche 454 technology. We ide ...[more]