Ontology highlight
ABSTRACT:
SUBMITTER: Burgtorf C
PROVIDER: S-EPMC403814 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Genome research 20031201 12
We present a novel methodology to determine the phase of single-nucleotide polymorphisms (SNPs) on a chromosome, which we term clone-based systematic haplotyping (CSH). The CSH procedure is based on separating the allelic chromosomes of a diploid genome by fosmid/cosmid cloning, and subsequent SNP typing of 96 clone pools, each representing approximately 10% of the genome. The pools are screened by PCR for the sequence of interest, followed by SNP typing on the PCR products using the GOOD assay. ...[more]