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New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.

ABSTRACT: The expression of certain genes involved in fundamental metabolism is regulated by metabolite-binding "riboswitch" elements embedded within their corresponding mRNAs. We have identified at least six additional elements within the Bacillus subtilis genome that exhibit characteristics of riboswitch function (glmS, gcvT, ydaO/yuaA, ykkC/yxkD, ykoK, and yybP/ykoY). These motifs exhibit extensive sequence and secondary-structure conservation among many bacterial species and occur upstream of related genes. The element located upstream of the glmS gene in Gram-positive organisms functions as a metabolite-dependent ribozyme that responds to glucosamine-6-phosphate. Other motifs form complex folded structures when transcribed as RNA molecules and carry intrinsic terminator structures. These findings indicate that riboswitches serve as a major genetic regulatory mechanism for the control of metabolic genes in many microbial species.

SUBMITTER: Barrick JE 

PROVIDER: S-EPMC404060 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

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New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.

Barrick Jeffrey E JE   Corbino Keith A KA   Winkler Wade C WC   Nahvi Ali A   Mandal Maumita M   Collins Jennifer J   Lee Mark M   Roth Adam A   Sudarsan Narasimhan N   Jona Inbal I   Wickiser J Kenneth JK   Breaker Ronald R RR  

Proceedings of the National Academy of Sciences of the United States of America 20040419 17

The expression of certain genes involved in fundamental metabolism is regulated by metabolite-binding "riboswitch" elements embedded within their corresponding mRNAs. We have identified at least six additional elements within the Bacillus subtilis genome that exhibit characteristics of riboswitch function (glmS, gcvT, ydaO/yuaA, ykkC/yxkD, ykoK, and yybP/ykoY). These motifs exhibit extensive sequence and secondary-structure conservation among many bacterial species and occur upstream of related  ...[more]

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