Ontology highlight
ABSTRACT:
SUBMITTER: Xie S
PROVIDER: S-EPMC4051533 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology communications 20140510 Pt 6
Uba5 is the smallest ubiquitin-like molecule-activating enzyme and contains an adenylation domain and a C-terminal region. This enzyme only exists in multicellular organisms. The mechanism through which the enzyme recognizes and activates ubiquitin-fold modifier 1 (Ufm1) remains unknown. In this study, Uba5 adenylation domains with different C-terminal region lengths were cloned, expressed and purified. The results of an in vitro truncation assay suggest that Uba5 residues 57-363 comprise the mi ...[more]