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A rapid, site-selective and efficient route to the dual modification of DARPins.

ABSTRACT: Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.

SUBMITTER: Moody P

PROVIDER: S-EPMC4091302 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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A rapid, site-selective and efficient route to the dual modification of DARPins.

Moody Paul P Chudasama Vijay V Nathani Ramiz I RI Maruani Antoine A Martin Stephen S Smith Mark E B ME Caddick Stephen S

Chemical communications (Cambridge, England) 20140501 38

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification. ...[more]

PMID: 24687090

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