Ontology highlight
ABSTRACT:
SUBMITTER: Nathani RI
PROVIDER: S-EPMC3985185 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Nathani Ramiz I RI Moody Paul P Chudasama Vijay V Smith Mark E B ME Fitzmaurice Richard J RJ Caddick Stephen S
Chemical science 20130618 9
Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling. ...[more]