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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification.


ABSTRACT: Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.

SUBMITTER: Nathani RI 

PROVIDER: S-EPMC3985185 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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A novel approach to the site-selective dual labelling of a protein <i>via</i> chemoselective cysteine modification.

Nathani Ramiz I RI   Moody Paul P   Chudasama Vijay V   Smith Mark E B ME   Fitzmaurice Richard J RJ   Caddick Stephen S  

Chemical science 20130618 9


Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling. ...[more]

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