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Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. • METHODS AND RESULTS:A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. • CONCLUSIONS:These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.

SUBMITTER: Ison JL

PROVIDER: S-EPMC4103461 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Ison Jennifer L JL Wagenius Stuart S Reitz Diedre D Ashley Mary V MV

Applications in plant sciences 20131107 11

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. •<h4>Methods and results</h4>A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populat ...[more]

PMID: 25202499

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