Dataset Information

Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. • METHODS AND RESULTS:A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. • CONCLUSIONS:These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.

SUBMITTER: Ison JL

PROVIDER: S-EPMC4103461 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Ison Jennifer L JL Wagenius Stuart S Reitz Diedre D Ashley Mary V MV

Applications in plant sciences 20131107 11

<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. •<h4>Methods and results</h4>A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populat ...[more]

PMID: 25202499

Similar Datasets

Project description:Backgrounds and aimsEchinacea angustifolia is a widespread species distributed throughout the Great Plains region of North America. Genetic differentiation among populations was investigated along a 1500 km north-south climatic gradient in North America, a region with no major geographical barriers. The objective of the study was to determine if genetic differentiation of populations could be explained by an isolation-by-distance model or by associations with climatic parameters known to affect plant growth and survival.MethodsHistorical climatic data were used to define the nature of the climatic gradient and AFLP markers were used to establish patterns of population genetic differentiation among ten Echinacea populations collected from North Dakota to Oklahoma. A total of 1290 fragments were scored using six EcoRI/MseI and three PstI/MseI primer combinations. Assessment of the correlation between climatic, genetic and geographic distances was assessed by Mantel and partial Mantel tests.Key resultsPstI/MseI combinations produced significantly fewer fragments, but a larger percentage was unique compared with EcoRI/MseI markers. Using estimates of F(ST), populations in Oklahoma and southern Kansas were identified as the most divergent from the other populations. Both the neighbour-joining tree and principal co-ordinate analysis clustered the populations in a north-south spatial orientation. About 60% of the genetic variation was found within populations, 20% among populations and the remaining 20% was partitioned among groups that were defined by the topology of the neighbour-joining tree. Significant support was found for the isolation-by-distance model independent of the effects of annual mean precipitation, but not from annual mean temperature and freeze-free days.ConclusionsEchinacea angustifolia populations exhibit genetic divergence along a north-south climatic gradient. The data support an isolation-by-distance restriction in gene flow that is independent of annual mean precipitation.

Dataset Information

Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Publications

Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets