Ontology highlight
ABSTRACT:
SUBMITTER: Xiao R
PROVIDER: S-EPMC4110633 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Xiao Rong R Anderson Stephen S Aramini James J Belote Rachel R Buchwald William A WA Ciccosanti Colleen C Conover Ken K Everett John K JK Hamilton Keith K Huang Yuanpeng Janet YJ Janjua Haleema H Jiang Mei M Kornhaber Gregory J GJ Lee Dong Yup DY Locke Jessica Y JY Ma Li-Chung LC Maglaqui Melissa M Mao Lei L Mitra Saheli S Patel Dayaban D Rossi Paolo P Sahdev Seema S Sharma Seema S Shastry Ritu R Swapna G V T GV Tong Saichu N SN Wang Dongyan D Wang Huang H Zhao Li L Montelione Gaetano T GT Acton Thomas B TB
Journal of structural biology 20100803 1
We describe the core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) and outline the strategies used for producing high-quality protein samples. The platform is centered on the cloning, expression and purification of 6X-His-tagged proteins using T7-based Escherichia coli systems. The 6X-His tag allows for similar purification procedures for most targets and implementation of high-throughput (HTP) parallel methods. In most cases, the 6X-His-tagged proteins are s ...[more]