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Clostridium difficile IStron CdISt1: discovery of a variant encoding two complete transposase-like proteins.

ABSTRACT: Screening a Clostridium difficile strain collection for the chimeric element CdISt1, we identified two additional variants, designated CdISt1-0 and CdISt1-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdISt1 variants led us to define four types of IStrons that we designated CdISt1-0 through CdISt1-III. Since CdISt1-0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.

SUBMITTER: Hasselmayer O

PROVIDER: S-EPMC412115 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

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Clostridium difficile IStron CdISt1: discovery of a variant encoding two complete transposase-like proteins.

Hasselmayer Oliver O Braun Veit V Nitsche Claudia C Moos Michael M Rupnik Maja M von Eichel-Streiber Christoph C

Journal of bacteriology 20040401 8

Screening a Clostridium difficile strain collection for the chimeric element CdISt1, we identified two additional variants, designated CdISt1-0 and CdISt1-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdISt1 variants led us to define four types of IStrons that we designated CdISt1-0 through CdISt1-III. Since CdISt1-0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the ...[more]

PMID: 15060058

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