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Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies.

ABSTRACT: We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.

SUBMITTER: Xi W

PROVIDER: S-EPMC4133182 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies.

Xi Wang W Schmidt Christine K CK Sanchez Samuel S Gracias David H DH Carazo-Salas Rafael E RE Jackson Stephen P SP Schmidt Oliver G OG

Nano letters 20140310 8

We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression ...[more]

PMID: 24598026

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