Ontology highlight
ABSTRACT:
SUBMITTER: Heckl D
PROVIDER: S-EPMC4160386 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Nature biotechnology 20140622 9
Genome sequencing studies have shown that human malignancies often bear mutations in four or more driver genes, but it is difficult to recapitulate this degree of genetic complexity in mouse models using conventional breeding. Here we use the CRISPR-Cas9 system of genome editing to overcome this limitation. By delivering combinations of small guide RNAs (sgRNAs) and Cas9 with a lentiviral vector, we modified up to five genes in a single mouse hematopoietic stem cell (HSC), leading to clonal outg ...[more]