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Next generation genetically encoded fluorescent sensors for serotonin.

ABSTRACT: We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

SUBMITTER: Kubitschke M

PROVIDER: S-EPMC9726753 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Next generation genetically encoded fluorescent sensors for serotonin.

Kubitschke Martin M Müller Monika M Wallhorn Lutz L Pulin Mauro M Mittag Manuel M Pollok Stefan S Ziebarth Tim T Bremshey Svenja S Gerdey Jill J Claussen Kristin Carolin KC Renken Kim K Groß Juliana J Gneiße Pascal P Meyer Niklas N Wiegert J Simon JS Reiner Andreas A Fuhrmann Martin M Masseck Olivia Andrea OA

Nature communications 20221206 1

We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal an ...[more]

PMID: 36473867

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