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The NF-?B regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity.


ABSTRACT: Bcl-3 is an atypical member of the I?B family and modulates gene expression via interaction with p50/NF-?B1 or p52/NF-?B2 homodimers. We report in the present study that Bcl-3 is required in dendritic cells (DCs) to assure effective priming of CD4 and CD8 T cells. Lack of Bcl-3 in bone marrow-derived DCs blunted their ability to expand and promote effector functions of T cells upon Ag/adjuvant challenge in vitro and after adoptive transfers in vivo. Importantly, the critical role of Bcl-3 for priming of T cells was exposed upon Ag/adjuvant challenge of mice specifically ablated of Bcl-3 in DCs. Furthermore, Bcl-3 in endogenous DCs was necessary for contact hypersensitivity responses. Bcl-3 modestly aided maturation of DCs, but most consequentially, Bcl-3 promoted their survival, partially inhibiting expression of several antiapoptotic genes. Loss of Bcl-3 accelerated apoptosis of bone marrow-derived DCs during Ag presentation to T cells, and DC survival was markedly impaired in the context of inflammatory conditions in mice specifically lacking Bcl-3 in these cells. Conversely, selective overexpression of Bcl-3 in DCs extended their lifespan in vitro and in vivo, correlating with increased capacity to prime T cells. These results expose a previously unidentified function for Bcl-3 in DC survival and the generation of adaptive immunity.

SUBMITTER: Tassi I 

PROVIDER: S-EPMC4201953 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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The NF-κB regulator Bcl-3 governs dendritic cell antigen presentation functions in adaptive immunity.

Tassi Ilaria I   Claudio Estefania E   Wang Hongshan H   Tang Wanhu W   Ha Hye-lin HL   Saret Sun S   Ramaswamy Madhu M   Siegel Richard R   Siebenlist Ulrich U  

Journal of immunology (Baltimore, Md. : 1950) 20140922 9


Bcl-3 is an atypical member of the IκB family and modulates gene expression via interaction with p50/NF-κB1 or p52/NF-κB2 homodimers. We report in the present study that Bcl-3 is required in dendritic cells (DCs) to assure effective priming of CD4 and CD8 T cells. Lack of Bcl-3 in bone marrow-derived DCs blunted their ability to expand and promote effector functions of T cells upon Ag/adjuvant challenge in vitro and after adoptive transfers in vivo. Importantly, the critical role of Bcl-3 for pr  ...[more]

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