Ontology highlight
ABSTRACT:
SUBMITTER: Cocucci E
PROVIDER: S-EPMC4230619 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Cocucci Emanuele E Gaudin Raphaël R Kirchhausen Tom T
Molecular biology of the cell 20140917 22
Dynamin, the GTPase required for clathrin-mediated endocytosis, is recruited to clathrin-coated pits in two sequential phases. The first is associated with coated pit maturation; the second, with fission of the membrane neck of a coated pit. Using gene-edited cells that express dynamin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and high temporal resolution, we detected the arrival of dynamin at coated pits and defined dynamin dimers as the preferre ...[more]