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Dual-sugar imaging using isonitrile and azido-based click chemistries.

ABSTRACT: We report the first account of metabolically labelling N-acetylglucosamine, in conjunction with either N-acetylgalactosamine or N-acetylmannosamine using a combination of isonitrile- and azide-based chemistries. With the appropriately labelled fluorescent probe molecules, that react with either the azido or isonitrile groups, the method enabled co-visualisation of cancer cell glycoproteins.

SUBMITTER: Wainman YA

PROVIDER: S-EPMC4246059 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Dual-sugar imaging using isonitrile and azido-based click chemistries.

Wainman Yéléna A YA Neves André A AA Stairs Shaun S Stöckmann Henning H Ireland-Zecchini Heather H Brindle Kevin M KM Leeper Finian J FJ

Organic & biomolecular chemistry 20131101 42

We report the first account of metabolically labelling N-acetylglucosamine, in conjunction with either N-acetylgalactosamine or N-acetylmannosamine using a combination of isonitrile- and azide-based chemistries. With the appropriately labelled fluorescent probe molecules, that react with either the azido or isonitrile groups, the method enabled co-visualisation of cancer cell glycoproteins. ...[more]

PMID: 24065211

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