Ontology highlight
ABSTRACT:
SUBMITTER: Sok D
PROVIDER: S-EPMC4267403 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Sok Devin D van Gils Marit J MJ Pauthner Matthias M Julien Jean-Philippe JP Saye-Francisco Karen L KL Hsueh Jessica J Briney Bryan B Lee Jeong Hyun JH Le Khoa M KM Lee Peter S PS Hua Yuanzi Y Seaman Michael S MS Moore John P JP Ward Andrew B AB Wilson Ian A IA Sanders Rogier W RW Burton Dennis R DR
Proceedings of the National Academy of Sciences of the United States of America 20141124 49
Broadly neutralizing antibodies (bnAbs) targeting the trimer apex of HIV envelope are favored candidates for vaccine design and immunotherapy because of their great neutralization breadth and potency. However, methods of isolating bnAbs against this site have been limited by the quaternary nature of the epitope region. Here we report the use of a recombinant HIV envelope trimer, BG505 SOSIP.664 gp140, as an affinity reagent to isolate quaternary-dependent bnAbs from the peripheral blood mononucl ...[more]