Enzymatic fabrication of high-density RNA arrays.
Ontology highlight
ABSTRACT:
SUBMITTER: Wu CH
PROVIDER: S-EPMC4319565 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Enzymatic fabrication of high-density RNA arrays.
Wu Cheng-Hsien CH Holden Matthew T MT Smith Lloyd M LM
Angewandte Chemie (International ed. in English) 20141022 49
A powerful new strategy for the fabrication of high-density RNA arrays is described. A high-density DNA array is fabricated by standard photolithographic methods, the surface-bound DNA molecules are enzymatically copied into their RNA complements from a surface-bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behind the desired RNA array. The strategy is compatible with 2'-fluoro-modified (2'F) ribonucleoside triphosphates (rNTPs), which may be included in the polymer ...[more]
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