Ontology highlight
ABSTRACT:
SUBMITTER: Liu Z
PROVIDER: S-EPMC4367261 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Liu Zhi Z Leng Esther C EC Gunasekaran Kannan K Pentony Martin M Shen Min M Howard Monique M Stoops Janelle J Manchulenko Kathy K Razinkov Vladimir V Liu Hua H Fanslow William W Hu Zhonghua Z Sun Nancy N Hasegawa Haruki H Clark Rutilio R Foltz Ian N IN Yan Wei W
The Journal of biological chemistry 20150112 12
Producing pure and well behaved bispecific antibodies (bsAbs) on a large scale for preclinical and clinical testing is a challenging task. Here, we describe a new strategy for making monovalent bispecific heterodimeric IgG antibodies in mammalian cells. We applied an electrostatic steering mechanism to engineer antibody light chain-heavy chain (LC-HC) interface residues in such a way that each LC strongly favors its cognate HC when two different HCs and two different LCs are co-expressed in the ...[more]