Ontology highlight
ABSTRACT:
SUBMITTER: Blanchet S
PROVIDER: S-EPMC4381064 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Blanchet Sandra S Rowe Michelle M Von der Haar Tobias T Fabret Céline C Demais Stéphane S Howard Mark J MJ Namy Olivier O
Nucleic acids research 20150303 6
In eukaryotes, translation termination is performed by eRF1, which recognizes stop codons via its N-terminal domain. Many previous studies based on point mutagenesis, cross-linking experiments or eRF1 chimeras have investigated the mechanism by which the stop signal is decoded by eRF1. Conserved motifs, such as GTS and YxCxxxF, were found to be important for termination efficiency, but the recognition mechanism remains unclear. We characterized a region of the eRF1 N-terminal domain, the P1 pock ...[more]