Ontology highlight
ABSTRACT:
SUBMITTER: Doran C
PROVIDER: S-EPMC4460156 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Doran Ciara C Chetrit Jonathan J Holley Matthew C MC Grundy David D Nassar Mohammed A MA
PloS one 20150608 6
In vitro cell lines from DRG neurons aid drug discovery because they can be used for early stage, high-throughput screens for drugs targeting pain pathways, with minimal dependence on animals. We have established a conditionally immortal DRG cell line from the Immortomouse. Using immunocytochemistry, RT-PCR and calcium microfluorimetry, we demonstrate that the cell line MED17.11 expresses markers of cells committed to the sensory neuron lineage. Within a few hours under differentiating condition ...[more]