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Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.

ABSTRACT: We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza virus HA immunogens.

SUBMITTER: Lee PS

PROVIDER: S-EPMC4473583 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer.

Lee Peter S PS Zhu Xueyong X Yu Wenli W Wilson Ian A IA

Journal of virology 20150429 14

We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, st ...[more]

PMID: 25926650

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