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40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer

ABSTRACT:

SUBMITTER: Yong Zi Tan

PROVIDER: EMPIAR-10097 | biostudies-other |

REPOSITORIES: biostudies-other

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Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

Tan Yong Zi YZ Baldwin Philip R PR Davis Joseph H JH Williamson James R JR Potter Clinton S CS Carragher Bridget B Lyumkis Dmitry D

Nature methods 20170703 8

We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adop ...[more]

PMID: 28671674

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