Ontology highlight
ABSTRACT:
SUBMITTER: Pulsipher KW
PROVIDER: S-EPMC6199156 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20181001 10
We characterize the encapsulation of supercharged green fluorescent protein, GFP(+36), by thermophilic ferritin from Archaeoglobus fulgidus (AfFtn). The AfFtn-GFP(+36) assembly is rapid, nearly stoichiometric, and robust. Using a more stably assembled mutant AfFtn, we show that encapsulation can occur in the presence of mostly assembled cages, in addition to encapsulation starting from AfFtn individual subunits. Assembly and encapsulation do not occur with non-supercharged GFP or the alternately ...[more]