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CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum.

ABSTRACT: Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. We used the CalFluor probes to image various alkyne-labeled biomolecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices.

SUBMITTER: Shieh P

PROVIDER: S-EPMC4487548 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible Spectrum.

Shieh Peyton P Dien Vivian T VT Beahm Brendan J BJ Castellano Joseph M JM Wyss-Coray Tony T Bertozzi Carolyn R CR

Journal of the American Chemical Society 20150602 22

Fluorescent bioorthogonal smart probes across the visible spectrum will enable sensitive visualization of metabolically labeled molecules in biological systems. Here we present a unified design, based on the principle of photoinduced electron transfer, to access a panel of highly fluorogenic azide probes that are activated by conversion to the corresponding triazoles via click chemistry. Termed the CalFluors, these probes possess emission maxima that range from green to far red wavelengths, and ...[more]

PMID: 25902190

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